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blocking antibodies against dnam-1 (cd226)  (Thermo Fisher)


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    Thermo Fisher blocking antibodies against dnam-1 (cd226)
    Expression analysis of NKC ligands on iPSC-CMs with or without IFN-γ treatment and expression of NKC-activating receptors on purified NKCs before coculture. ( A ) Flow cytometric detection of MHC class I molecule expression on C57BL/6 splenocytes using antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( B ) Flow cytometric detection of MHC class I molecule expression on iPSC-CMs with or without IFN-γ treatment. The iPSC-CMs were stained with antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( C ) Flow cytometric detection of NKC-activating ligands on iPSC-CMs at day 16. The iPSC-CMs were stained with antibodies against MULT1, H-60, RAE-1, CD112, and CD155, or isotype-matched control antibodies. ( D ) Flow cytometric detection of MHC class II (I-A/I-E) molecules on iPSC-CMs with or without IFN-γ treatment, stained with anti-I-A/I-E, CD40, CD80, and CD86 antibodies or isotype-matched control antibodies. ( E ) mRNA expression of CIITA and NLRC5 in iPSC-CMs with or without IFN-γ treatment, as measured by real-time PCR. The results are expressed relative to those of C57BL/6 splenocytes as means ± SD (n = 3, respectively); *p < 0.05. ( F ) Flow cytometric detection of NK1.1 and CD3 expression on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against NK1.1 and CD3, or isotype-matched control antibodies. ( G ) Flow cytometric detection of NKC-activating receptors on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against <t>CD226</t> and NKG2D, or isotype-matched control antibodies.
    Blocking Antibodies Against Dnam 1 (Cd226), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+antibodies+against+dnam-1+%28cd226%29/pmc06658523-179-8-12?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    blocking antibodies against dnam-1 (cd226) - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Natural killer cells impede the engraftment of cardiomyocytes derived from induced pluripotent stem cells in syngeneic mouse model"

    Article Title: Natural killer cells impede the engraftment of cardiomyocytes derived from induced pluripotent stem cells in syngeneic mouse model

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-47134-3

    Expression analysis of NKC ligands on iPSC-CMs with or without IFN-γ treatment and expression of NKC-activating receptors on purified NKCs before coculture. ( A ) Flow cytometric detection of MHC class I molecule expression on C57BL/6 splenocytes using antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( B ) Flow cytometric detection of MHC class I molecule expression on iPSC-CMs with or without IFN-γ treatment. The iPSC-CMs were stained with antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( C ) Flow cytometric detection of NKC-activating ligands on iPSC-CMs at day 16. The iPSC-CMs were stained with antibodies against MULT1, H-60, RAE-1, CD112, and CD155, or isotype-matched control antibodies. ( D ) Flow cytometric detection of MHC class II (I-A/I-E) molecules on iPSC-CMs with or without IFN-γ treatment, stained with anti-I-A/I-E, CD40, CD80, and CD86 antibodies or isotype-matched control antibodies. ( E ) mRNA expression of CIITA and NLRC5 in iPSC-CMs with or without IFN-γ treatment, as measured by real-time PCR. The results are expressed relative to those of C57BL/6 splenocytes as means ± SD (n = 3, respectively); *p < 0.05. ( F ) Flow cytometric detection of NK1.1 and CD3 expression on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against NK1.1 and CD3, or isotype-matched control antibodies. ( G ) Flow cytometric detection of NKC-activating receptors on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against CD226 and NKG2D, or isotype-matched control antibodies.
    Figure Legend Snippet: Expression analysis of NKC ligands on iPSC-CMs with or without IFN-γ treatment and expression of NKC-activating receptors on purified NKCs before coculture. ( A ) Flow cytometric detection of MHC class I molecule expression on C57BL/6 splenocytes using antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( B ) Flow cytometric detection of MHC class I molecule expression on iPSC-CMs with or without IFN-γ treatment. The iPSC-CMs were stained with antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( C ) Flow cytometric detection of NKC-activating ligands on iPSC-CMs at day 16. The iPSC-CMs were stained with antibodies against MULT1, H-60, RAE-1, CD112, and CD155, or isotype-matched control antibodies. ( D ) Flow cytometric detection of MHC class II (I-A/I-E) molecules on iPSC-CMs with or without IFN-γ treatment, stained with anti-I-A/I-E, CD40, CD80, and CD86 antibodies or isotype-matched control antibodies. ( E ) mRNA expression of CIITA and NLRC5 in iPSC-CMs with or without IFN-γ treatment, as measured by real-time PCR. The results are expressed relative to those of C57BL/6 splenocytes as means ± SD (n = 3, respectively); *p < 0.05. ( F ) Flow cytometric detection of NK1.1 and CD3 expression on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against NK1.1 and CD3, or isotype-matched control antibodies. ( G ) Flow cytometric detection of NKC-activating receptors on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against CD226 and NKG2D, or isotype-matched control antibodies.

    Techniques Used: Expressing, Purification, Control, Staining, Real-time Polymerase Chain Reaction, Cell Culture



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    Expression analysis of NKC ligands on iPSC-CMs with or without IFN-γ treatment and expression of NKC-activating receptors on purified NKCs before coculture. ( A ) Flow cytometric detection of MHC class I molecule expression on C57BL/6 splenocytes using antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( B ) Flow cytometric detection of MHC class I molecule expression on iPSC-CMs with or without IFN-γ treatment. The iPSC-CMs were stained with antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( C ) Flow cytometric detection of NKC-activating ligands on iPSC-CMs at day 16. The iPSC-CMs were stained with antibodies against MULT1, H-60, RAE-1, CD112, and CD155, or isotype-matched control antibodies. ( D ) Flow cytometric detection of MHC class II (I-A/I-E) molecules on iPSC-CMs with or without IFN-γ treatment, stained with anti-I-A/I-E, CD40, CD80, and CD86 antibodies or isotype-matched control antibodies. ( E ) mRNA expression of CIITA and NLRC5 in iPSC-CMs with or without IFN-γ treatment, as measured by real-time PCR. The results are expressed relative to those of C57BL/6 splenocytes as means ± SD (n = 3, respectively); *p < 0.05. ( F ) Flow cytometric detection of NK1.1 and CD3 expression on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against NK1.1 and CD3, or isotype-matched control antibodies. ( G ) Flow cytometric detection of NKC-activating receptors on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against <t>CD226</t> and NKG2D, or isotype-matched control antibodies.
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    Expression analysis of NKC ligands on iPSC-CMs with or without IFN-γ treatment and expression of NKC-activating receptors on purified NKCs before coculture. ( A ) Flow cytometric detection of MHC class I molecule expression on C57BL/6 splenocytes using antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( B ) Flow cytometric detection of MHC class I molecule expression on iPSC-CMs with or without IFN-γ treatment. The iPSC-CMs were stained with antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( C ) Flow cytometric detection of NKC-activating ligands on iPSC-CMs at day 16. The iPSC-CMs were stained with antibodies against MULT1, H-60, RAE-1, CD112, and CD155, or isotype-matched control antibodies. ( D ) Flow cytometric detection of MHC class II (I-A/I-E) molecules on iPSC-CMs with or without IFN-γ treatment, stained with anti-I-A/I-E, CD40, CD80, and CD86 antibodies or isotype-matched control antibodies. ( E ) mRNA expression of CIITA and NLRC5 in iPSC-CMs with or without IFN-γ treatment, as measured by real-time PCR. The results are expressed relative to those of C57BL/6 splenocytes as means ± SD (n = 3, respectively); *p < 0.05. ( F ) Flow cytometric detection of NK1.1 and CD3 expression on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against NK1.1 and CD3, or isotype-matched control antibodies. ( G ) Flow cytometric detection of NKC-activating receptors on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against <t>CD226</t> and NKG2D, or isotype-matched control antibodies.
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    Image Search Results


    Expression analysis of NKC ligands on iPSC-CMs with or without IFN-γ treatment and expression of NKC-activating receptors on purified NKCs before coculture. ( A ) Flow cytometric detection of MHC class I molecule expression on C57BL/6 splenocytes using antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( B ) Flow cytometric detection of MHC class I molecule expression on iPSC-CMs with or without IFN-γ treatment. The iPSC-CMs were stained with antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( C ) Flow cytometric detection of NKC-activating ligands on iPSC-CMs at day 16. The iPSC-CMs were stained with antibodies against MULT1, H-60, RAE-1, CD112, and CD155, or isotype-matched control antibodies. ( D ) Flow cytometric detection of MHC class II (I-A/I-E) molecules on iPSC-CMs with or without IFN-γ treatment, stained with anti-I-A/I-E, CD40, CD80, and CD86 antibodies or isotype-matched control antibodies. ( E ) mRNA expression of CIITA and NLRC5 in iPSC-CMs with or without IFN-γ treatment, as measured by real-time PCR. The results are expressed relative to those of C57BL/6 splenocytes as means ± SD (n = 3, respectively); *p < 0.05. ( F ) Flow cytometric detection of NK1.1 and CD3 expression on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against NK1.1 and CD3, or isotype-matched control antibodies. ( G ) Flow cytometric detection of NKC-activating receptors on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against CD226 and NKG2D, or isotype-matched control antibodies.

    Journal: Scientific Reports

    Article Title: Natural killer cells impede the engraftment of cardiomyocytes derived from induced pluripotent stem cells in syngeneic mouse model

    doi: 10.1038/s41598-019-47134-3

    Figure Lengend Snippet: Expression analysis of NKC ligands on iPSC-CMs with or without IFN-γ treatment and expression of NKC-activating receptors on purified NKCs before coculture. ( A ) Flow cytometric detection of MHC class I molecule expression on C57BL/6 splenocytes using antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( B ) Flow cytometric detection of MHC class I molecule expression on iPSC-CMs with or without IFN-γ treatment. The iPSC-CMs were stained with antibodies against H2Db, H2Kb, Qa1-b, and Qa2, or isotype-matched control antibodies. ( C ) Flow cytometric detection of NKC-activating ligands on iPSC-CMs at day 16. The iPSC-CMs were stained with antibodies against MULT1, H-60, RAE-1, CD112, and CD155, or isotype-matched control antibodies. ( D ) Flow cytometric detection of MHC class II (I-A/I-E) molecules on iPSC-CMs with or without IFN-γ treatment, stained with anti-I-A/I-E, CD40, CD80, and CD86 antibodies or isotype-matched control antibodies. ( E ) mRNA expression of CIITA and NLRC5 in iPSC-CMs with or without IFN-γ treatment, as measured by real-time PCR. The results are expressed relative to those of C57BL/6 splenocytes as means ± SD (n = 3, respectively); *p < 0.05. ( F ) Flow cytometric detection of NK1.1 and CD3 expression on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against NK1.1 and CD3, or isotype-matched control antibodies. ( G ) Flow cytometric detection of NKC-activating receptors on cultured NKCs purified from C57BL/6 splenocytes, stained with antibodies against CD226 and NKG2D, or isotype-matched control antibodies.

    Article Snippet: Cultured NKCs were washed and incubated with blocking antibodies against DNAM-1 (CD226) (Thermo Fisher Scientific) and NKG2D (BioLegend) for 90 min at room temperature.

    Techniques: Expressing, Purification, Control, Staining, Real-time Polymerase Chain Reaction, Cell Culture